growth differentiation factor 15 Search Results


recombinant mature gdf9  (MedChemExpress)
92
MedChemExpress recombinant mature gdf9
Recombinant Mature Gdf9, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bt lab cat no e2043hu bt lab cat no e0090hu  (Shanghai Korain Biotech Co Ltd)
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Shanghai Korain Biotech Co Ltd bt lab cat no e2043hu bt lab cat no e0090hu
Bt Lab Cat No E2043hu Bt Lab Cat No E0090hu, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti il 6  (Proteintech)
96
Proteintech anti il 6
Anti Il 6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il6  (Proteintech)
93
Proteintech human il6
Human Il6, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il 6 elisa kit  (Proteintech)
94
Proteintech human il 6 elisa kit
Human Il 6 Elisa Kit, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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control rabbit immunoglobulin  (ProSci Incorporated)
90
ProSci Incorporated control rabbit immunoglobulin
A): Map of lentiviral RASSF1A construct. B): ACHN cells were transduced with RASSF1A carrying lentivirus or empty virus. 72h later selective antibiotic was added and the population of clones was grown in selection media for 14 days before assessing apoptotic response to IFN-β (50 U/ml over 5 days) by TUNEL assay. After confirmation of stable expression of RASSF1A by immunoblotting, clonal selection was performed to identify clones that expressed similar amounts of RASSF1A protein as achieved by DNMT1 AS (40 nM) over 8 days. Treatment with DNMT1 mismatch control oligonucleotide (MM) and empty lentivirus transduced cells served as negative controls. In clones expressing RASSF1A at levels comparable to DNA demethylating treatment, IFN-β (50 U/ml over 5 days) resulted in 20–40% apoptotic cells while stable transduction with empty lentivirus (kept in selective antibiotic for equal amounts of time) did not. C): Concurrent treatment of ACHN RASSF1A clone 1.3 with 2 μg/ml TRAIL neutralizing antibody (TRAIL-N AB) and IFN-β (50 U/ml) over 5 days inhibited IFN-induced apoptosis compared to cotreatment with 2 μg/ml control rabbit <t>immunoglobulin</t> (CTRL AB). D): RASSF1A expression markedly increased sensitivity to apoptosis induction by Apo2L/TRAIL in ACHN cells. Normal kidney epithelial (NKE) cells, even after pretreatment with 5-AZA-dC (200 nM) over 4 days, which alone resulted in moderate apoptosis induction, remained resistant to the apoptosis inducing effects of Apo2L/TRAIL. TUNEL graphs display the means and standard deviations (error bars) of independent experiments.
Control Rabbit Immunoglobulin, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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release buffer  (Boster Bio)
92
Boster Bio release buffer
A): Map of lentiviral RASSF1A construct. B): ACHN cells were transduced with RASSF1A carrying lentivirus or empty virus. 72h later selective antibiotic was added and the population of clones was grown in selection media for 14 days before assessing apoptotic response to IFN-β (50 U/ml over 5 days) by TUNEL assay. After confirmation of stable expression of RASSF1A by immunoblotting, clonal selection was performed to identify clones that expressed similar amounts of RASSF1A protein as achieved by DNMT1 AS (40 nM) over 8 days. Treatment with DNMT1 mismatch control oligonucleotide (MM) and empty lentivirus transduced cells served as negative controls. In clones expressing RASSF1A at levels comparable to DNA demethylating treatment, IFN-β (50 U/ml over 5 days) resulted in 20–40% apoptotic cells while stable transduction with empty lentivirus (kept in selective antibiotic for equal amounts of time) did not. C): Concurrent treatment of ACHN RASSF1A clone 1.3 with 2 μg/ml TRAIL neutralizing antibody (TRAIL-N AB) and IFN-β (50 U/ml) over 5 days inhibited IFN-induced apoptosis compared to cotreatment with 2 μg/ml control rabbit <t>immunoglobulin</t> (CTRL AB). D): RASSF1A expression markedly increased sensitivity to apoptosis induction by Apo2L/TRAIL in ACHN cells. Normal kidney epithelial (NKE) cells, even after pretreatment with 5-AZA-dC (200 nM) over 4 days, which alone resulted in moderate apoptosis induction, remained resistant to the apoptosis inducing effects of Apo2L/TRAIL. TUNEL graphs display the means and standard deviations (error bars) of independent experiments.
Release Buffer, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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il 4  (Boster Bio)
90
Boster Bio il 4
A): Map of lentiviral RASSF1A construct. B): ACHN cells were transduced with RASSF1A carrying lentivirus or empty virus. 72h later selective antibiotic was added and the population of clones was grown in selection media for 14 days before assessing apoptotic response to IFN-β (50 U/ml over 5 days) by TUNEL assay. After confirmation of stable expression of RASSF1A by immunoblotting, clonal selection was performed to identify clones that expressed similar amounts of RASSF1A protein as achieved by DNMT1 AS (40 nM) over 8 days. Treatment with DNMT1 mismatch control oligonucleotide (MM) and empty lentivirus transduced cells served as negative controls. In clones expressing RASSF1A at levels comparable to DNA demethylating treatment, IFN-β (50 U/ml over 5 days) resulted in 20–40% apoptotic cells while stable transduction with empty lentivirus (kept in selective antibiotic for equal amounts of time) did not. C): Concurrent treatment of ACHN RASSF1A clone 1.3 with 2 μg/ml TRAIL neutralizing antibody (TRAIL-N AB) and IFN-β (50 U/ml) over 5 days inhibited IFN-induced apoptosis compared to cotreatment with 2 μg/ml control rabbit <t>immunoglobulin</t> (CTRL AB). D): RASSF1A expression markedly increased sensitivity to apoptosis induction by Apo2L/TRAIL in ACHN cells. Normal kidney epithelial (NKE) cells, even after pretreatment with 5-AZA-dC (200 nM) over 4 days, which alone resulted in moderate apoptosis induction, remained resistant to the apoptosis inducing effects of Apo2L/TRAIL. TUNEL graphs display the means and standard deviations (error bars) of independent experiments.
Il 4, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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il 6  (Boster Bio)
95
Boster Bio il 6
A): Map of lentiviral RASSF1A construct. B): ACHN cells were transduced with RASSF1A carrying lentivirus or empty virus. 72h later selective antibiotic was added and the population of clones was grown in selection media for 14 days before assessing apoptotic response to IFN-β (50 U/ml over 5 days) by TUNEL assay. After confirmation of stable expression of RASSF1A by immunoblotting, clonal selection was performed to identify clones that expressed similar amounts of RASSF1A protein as achieved by DNMT1 AS (40 nM) over 8 days. Treatment with DNMT1 mismatch control oligonucleotide (MM) and empty lentivirus transduced cells served as negative controls. In clones expressing RASSF1A at levels comparable to DNA demethylating treatment, IFN-β (50 U/ml over 5 days) resulted in 20–40% apoptotic cells while stable transduction with empty lentivirus (kept in selective antibiotic for equal amounts of time) did not. C): Concurrent treatment of ACHN RASSF1A clone 1.3 with 2 μg/ml TRAIL neutralizing antibody (TRAIL-N AB) and IFN-β (50 U/ml) over 5 days inhibited IFN-induced apoptosis compared to cotreatment with 2 μg/ml control rabbit <t>immunoglobulin</t> (CTRL AB). D): RASSF1A expression markedly increased sensitivity to apoptosis induction by Apo2L/TRAIL in ACHN cells. Normal kidney epithelial (NKE) cells, even after pretreatment with 5-AZA-dC (200 nM) over 4 days, which alone resulted in moderate apoptosis induction, remained resistant to the apoptosis inducing effects of Apo2L/TRAIL. TUNEL graphs display the means and standard deviations (error bars) of independent experiments.
Il 6, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 6 - by Bioz Stars, 2026-03
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mouse monoclonal antibody against nrg1  (Proteintech)
94
Proteintech mouse monoclonal antibody against nrg1
A): Map of lentiviral RASSF1A construct. B): ACHN cells were transduced with RASSF1A carrying lentivirus or empty virus. 72h later selective antibiotic was added and the population of clones was grown in selection media for 14 days before assessing apoptotic response to IFN-β (50 U/ml over 5 days) by TUNEL assay. After confirmation of stable expression of RASSF1A by immunoblotting, clonal selection was performed to identify clones that expressed similar amounts of RASSF1A protein as achieved by DNMT1 AS (40 nM) over 8 days. Treatment with DNMT1 mismatch control oligonucleotide (MM) and empty lentivirus transduced cells served as negative controls. In clones expressing RASSF1A at levels comparable to DNA demethylating treatment, IFN-β (50 U/ml over 5 days) resulted in 20–40% apoptotic cells while stable transduction with empty lentivirus (kept in selective antibiotic for equal amounts of time) did not. C): Concurrent treatment of ACHN RASSF1A clone 1.3 with 2 μg/ml TRAIL neutralizing antibody (TRAIL-N AB) and IFN-β (50 U/ml) over 5 days inhibited IFN-induced apoptosis compared to cotreatment with 2 μg/ml control rabbit <t>immunoglobulin</t> (CTRL AB). D): RASSF1A expression markedly increased sensitivity to apoptosis induction by Apo2L/TRAIL in ACHN cells. Normal kidney epithelial (NKE) cells, even after pretreatment with 5-AZA-dC (200 nM) over 4 days, which alone resulted in moderate apoptosis induction, remained resistant to the apoptosis inducing effects of Apo2L/TRAIL. TUNEL graphs display the means and standard deviations (error bars) of independent experiments.
Mouse Monoclonal Antibody Against Nrg1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal antibody against nrg1 - by Bioz Stars, 2026-03
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murine interleukin 4 il 4  (MedChemExpress)
93
MedChemExpress murine interleukin 4 il 4
A): Map of lentiviral RASSF1A construct. B): ACHN cells were transduced with RASSF1A carrying lentivirus or empty virus. 72h later selective antibiotic was added and the population of clones was grown in selection media for 14 days before assessing apoptotic response to IFN-β (50 U/ml over 5 days) by TUNEL assay. After confirmation of stable expression of RASSF1A by immunoblotting, clonal selection was performed to identify clones that expressed similar amounts of RASSF1A protein as achieved by DNMT1 AS (40 nM) over 8 days. Treatment with DNMT1 mismatch control oligonucleotide (MM) and empty lentivirus transduced cells served as negative controls. In clones expressing RASSF1A at levels comparable to DNA demethylating treatment, IFN-β (50 U/ml over 5 days) resulted in 20–40% apoptotic cells while stable transduction with empty lentivirus (kept in selective antibiotic for equal amounts of time) did not. C): Concurrent treatment of ACHN RASSF1A clone 1.3 with 2 μg/ml TRAIL neutralizing antibody (TRAIL-N AB) and IFN-β (50 U/ml) over 5 days inhibited IFN-induced apoptosis compared to cotreatment with 2 μg/ml control rabbit <t>immunoglobulin</t> (CTRL AB). D): RASSF1A expression markedly increased sensitivity to apoptosis induction by Apo2L/TRAIL in ACHN cells. Normal kidney epithelial (NKE) cells, even after pretreatment with 5-AZA-dC (200 nM) over 4 days, which alone resulted in moderate apoptosis induction, remained resistant to the apoptosis inducing effects of Apo2L/TRAIL. TUNEL graphs display the means and standard deviations (error bars) of independent experiments.
Murine Interleukin 4 Il 4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
murine interleukin 4 il 4 - by Bioz Stars, 2026-03
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serum gdf 15  (Boster Bio)
93
Boster Bio serum gdf 15
A): Map of lentiviral RASSF1A construct. B): ACHN cells were transduced with RASSF1A carrying lentivirus or empty virus. 72h later selective antibiotic was added and the population of clones was grown in selection media for 14 days before assessing apoptotic response to IFN-β (50 U/ml over 5 days) by TUNEL assay. After confirmation of stable expression of RASSF1A by immunoblotting, clonal selection was performed to identify clones that expressed similar amounts of RASSF1A protein as achieved by DNMT1 AS (40 nM) over 8 days. Treatment with DNMT1 mismatch control oligonucleotide (MM) and empty lentivirus transduced cells served as negative controls. In clones expressing RASSF1A at levels comparable to DNA demethylating treatment, IFN-β (50 U/ml over 5 days) resulted in 20–40% apoptotic cells while stable transduction with empty lentivirus (kept in selective antibiotic for equal amounts of time) did not. C): Concurrent treatment of ACHN RASSF1A clone 1.3 with 2 μg/ml TRAIL neutralizing antibody (TRAIL-N AB) and IFN-β (50 U/ml) over 5 days inhibited IFN-induced apoptosis compared to cotreatment with 2 μg/ml control rabbit <t>immunoglobulin</t> (CTRL AB). D): RASSF1A expression markedly increased sensitivity to apoptosis induction by Apo2L/TRAIL in ACHN cells. Normal kidney epithelial (NKE) cells, even after pretreatment with 5-AZA-dC (200 nM) over 4 days, which alone resulted in moderate apoptosis induction, remained resistant to the apoptosis inducing effects of Apo2L/TRAIL. TUNEL graphs display the means and standard deviations (error bars) of independent experiments.
Serum Gdf 15, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A): Map of lentiviral RASSF1A construct. B): ACHN cells were transduced with RASSF1A carrying lentivirus or empty virus. 72h later selective antibiotic was added and the population of clones was grown in selection media for 14 days before assessing apoptotic response to IFN-β (50 U/ml over 5 days) by TUNEL assay. After confirmation of stable expression of RASSF1A by immunoblotting, clonal selection was performed to identify clones that expressed similar amounts of RASSF1A protein as achieved by DNMT1 AS (40 nM) over 8 days. Treatment with DNMT1 mismatch control oligonucleotide (MM) and empty lentivirus transduced cells served as negative controls. In clones expressing RASSF1A at levels comparable to DNA demethylating treatment, IFN-β (50 U/ml over 5 days) resulted in 20–40% apoptotic cells while stable transduction with empty lentivirus (kept in selective antibiotic for equal amounts of time) did not. C): Concurrent treatment of ACHN RASSF1A clone 1.3 with 2 μg/ml TRAIL neutralizing antibody (TRAIL-N AB) and IFN-β (50 U/ml) over 5 days inhibited IFN-induced apoptosis compared to cotreatment with 2 μg/ml control rabbit immunoglobulin (CTRL AB). D): RASSF1A expression markedly increased sensitivity to apoptosis induction by Apo2L/TRAIL in ACHN cells. Normal kidney epithelial (NKE) cells, even after pretreatment with 5-AZA-dC (200 nM) over 4 days, which alone resulted in moderate apoptosis induction, remained resistant to the apoptosis inducing effects of Apo2L/TRAIL. TUNEL graphs display the means and standard deviations (error bars) of independent experiments.

Journal:

Article Title: Expression of RASSF1A, an epigenetically silenced tumor suppressor, overcomes resistance to apoptosis induction by interferons

doi: 10.1158/0008-5472.CAN-05-2303

Figure Lengend Snippet: A): Map of lentiviral RASSF1A construct. B): ACHN cells were transduced with RASSF1A carrying lentivirus or empty virus. 72h later selective antibiotic was added and the population of clones was grown in selection media for 14 days before assessing apoptotic response to IFN-β (50 U/ml over 5 days) by TUNEL assay. After confirmation of stable expression of RASSF1A by immunoblotting, clonal selection was performed to identify clones that expressed similar amounts of RASSF1A protein as achieved by DNMT1 AS (40 nM) over 8 days. Treatment with DNMT1 mismatch control oligonucleotide (MM) and empty lentivirus transduced cells served as negative controls. In clones expressing RASSF1A at levels comparable to DNA demethylating treatment, IFN-β (50 U/ml over 5 days) resulted in 20–40% apoptotic cells while stable transduction with empty lentivirus (kept in selective antibiotic for equal amounts of time) did not. C): Concurrent treatment of ACHN RASSF1A clone 1.3 with 2 μg/ml TRAIL neutralizing antibody (TRAIL-N AB) and IFN-β (50 U/ml) over 5 days inhibited IFN-induced apoptosis compared to cotreatment with 2 μg/ml control rabbit immunoglobulin (CTRL AB). D): RASSF1A expression markedly increased sensitivity to apoptosis induction by Apo2L/TRAIL in ACHN cells. Normal kidney epithelial (NKE) cells, even after pretreatment with 5-AZA-dC (200 nM) over 4 days, which alone resulted in moderate apoptosis induction, remained resistant to the apoptosis inducing effects of Apo2L/TRAIL. TUNEL graphs display the means and standard deviations (error bars) of independent experiments.

Article Snippet: Apo2L / TRAIL was obtained from PeproTech (Rocky Hill, NJ), rabbit polyclonal TRAIL neutralizing antibody and control rabbit immunoglobulin from ProSci (Poway, CA).

Techniques: Construct, Transduction, Virus, Clone Assay, Selection, TUNEL Assay, Expressing, Western Blot, Control